The availability of the genome sequence provides the opportunity for rapid genotyping using the whole genome. The edible white rot fungus lentinula edodes possesses a variety of lignin degrading enzymes such as manganese peroxidases and laccases. Tree search blast pfam domains secondary metabolism clusters cazymes peptidases transporters transcription factors mcl clusters download nominate new species aaosphaeria arxii cbs 175. Highlights we firstly got lentinula edodes c 9 transcriptome by solexa sequencing successfully. Grouping of multicopper oxidases in lentinula edodes by. Comparative transcriptome analysis identified candidate genes. Lentinula edodes shiitake mushroom lentinus edodes uniprot. Genomic survey of lentinula genus doe joint genome institute. Genome sequence data of lentinula edodes was not available for pcr subtraction, yet approximately 50 genes, such as putative chitinases chi1. Lentinula edodes is a popular, cultivated edible and medicinal mushroom. Genome sequence of the cauliflower mushroom sparassis. Today, shiitake is cultivated worldwide, and it is second only to the.
In order to allow comparative analyses with other fungal genomes sequenced by the jgi, a copy of this genome. Lentinula edodes, the popular shiitake mushroom, is one of the most important cultivated edible mushrooms. Genome sequence and genetic linkage analysis of shiitake mushroom lentinula edodes. This is an invaluable resource for functional genomics studies in lentinula edodes.
Lentinula is a group of whiterot, wooddecaying fungi that is famous as the genus of shiitake mushrooms, lentinula edodes. Ddbj released genome sequence data of shiitake mushroom, lentinula edodes, which had been submitted by iwate biotechnology research center. In a single run, we produced more than 57 million sequencing reads. Sequence analysis and expression of a bluelight photoreceptor gene, le. Here we report finding of new alleles of pheromones phbs and pheromone receptors rcbs to constitute five alleles of rcb1 rcb11. Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruitingbody formation and lignin degradation.
Sequence characterized amplified region markers tightly. Lentinula edodes has been cultivated in china for about 800 years. As such, the genome sequence data processing and integration activities of its. Lentinula edodes is susceptible to postharvest problems, such as gill browning, fruiting body softening, and lentinan degradation. These reads were assembled into 28,923 unigene sequences mean size689bp including 18,120 unigenes with coding sequence. Genome sequence of the edible cultivated mushroom lentinula. As a white rot fungus with lignocellulose degradation ability, l. Lentinula edodes is one of the most popular edible mushrooms worldwide and contains important medicinal components such as lentinan, ergosterol, and eritadenine. The doe jgi is the global leader in generating genome sequences of plants, fungi, microbes, and metagenomes. Here, we identified the a matingtype locus from the first draft genome sequence of l. Genome sequence and genetic linkage analysis of shiitake.
Now in this shiitakegdb, you can use the nuclear genome gbrowse2 and blast tools or download genome data. Lentinula edodes genome sequencing consortium sakamoto y. Molecular characterization of a novel mycovirus in the. Lentinula edodes genome survey and postharvest transcriptome.
Jgi tools were used to automatically annotate predicted proteins. Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many asian countries, especially in china, japan and korea. Cloning and characterization of two hydrophobin genes. Heterologous expression of the pleurotus ostreatus mnp3 gene by the laccase gene promoter in lentinula edodes toshitsugu sato1,2, toshikazu irie1,3 and fumihiko yoshino3 1iwate biotechnology research center. Lentinula edodes, also known as xianggu or shiitake, belonging to the agaricales order of the agaricomycetes. Lentinula edodes, one of the most popular edible mushroom species with high nutrition and special flavor, is widely cultivated in many asian countries, especially in china, japan and korea. Hoi shan kwan 1, chun hang au 1, man chun wong 1, jing qin 1, iris sze wai kwok 1, winnie wing yan chum 1, pui. In lentinula edodes, multiple b mating types have been suggested based on genome sequence analysis and mating assay. It was discovered that hypervariable sequence region with an. Comparative transcriptome analysis identified candidate. Lentinula edodes, one of the most popular, edible mushroom species. Four primers, two sets of primers, were designed according to the internal sequences of two rapds tightly linking to the a factor or b factor. Sequencing revealed the actual length of isp aaagag450 to be 473 bp. Longread transcriptome data for improved gene prediction.
Over,000 gene models were predicted from the genome sequence. Genome sequence and genetic linkage analysis of shiitake mushroom lentinula edodes article pdf available in nature precedings january 2012 with 224 reads how we measure reads. The genome sequence and gene models of lentinula edodes b17 were not determined by the joint genome institute jgi, but were downloaded from ncbi and the article, park et al. Population genomic analysis uncovers environmental stress. Distribution of genes in the mata and matb loci of lentinula edodes. In order to allow comparative analyses with other fungal genomes sequenced by the jgi, a copy of this genome is incorporated into mycocosm. Heterologous expression of the pleurotus ostreatus mnp3. In the 1970s, mycoviruses were identified that infected the edible mushroom lentinula edodes shiitake, but they were not regarded as causal agents for mushroom diseases. The transcriptome included lots of unigene sequences and functional annotation. The fragment, isp aaagag450, was cloned and the nucleotide sequences of both strands of the dna determined. Lentinula edodes is one of the most popular edible mushrooms in the world and contains useful medicinal components such as lentinan.
Lentinula edodes, also known as the shiitake mushroom, is the third most commonly produced type of mushroom, following agaricus and. The genetic structure of the a matingtype locus of. Pdf diversity of a mating type in lentinula edodes and. Genome sequence of the edible cultivated mushroom lentinula edodes shiitake reveals insights into lignocellulose degradation. Wang y and peng y 2020 a resequencingbased ultradense genetic map of hericium erinaceus for anchoring genome sequences. Pdf genome sequence of the edible cultivated mushroom. Laccases belong to the multicopper oxidases, which have a wide range of catalytic activities including polyphenol degradation and synthesis, lignin degradation, and melanin formation. The genome sequence and gene models of lentinula edodes w126 were not determined by the joint genome institute jgi, but were downloaded from.
Transcriptome analysis of the edible mushroom lentinula. The genome sequence and gene models of lentinula edodes b17 were not determined by the joint genome institute jgi, but were downloaded from ncbi. The lightinduced formation of brown film on the vegetative mycelial tissues of l. Download table general features of the lentinula edodes genome. Use of intersimple sequence repeats markers to develop. Frontiers a resequencingbased ultradense genetic map of. Genome sequence of the edible cultivated mushroom lentinula edodes shiitake reveals insights into lignocellulose degradation article pdf available in plos one 118 august 2016 with 489 reads. Lentinula edodes is an edible mushroom native to east asia, which is cultivated and consumed in many asian countries. Identifying the matingtypes in shiitake is important for enhancing the breeding and cultivation of this economicallyimportant edible mushroom. Genome sequence data of lentinula edodes was not available for pcr subtraction, yet. Proteomes lentinula edodes shiitake mushroom lentinus edodes. Pheromone and pheromone receptor genes in b mating type locus constitute b mating type of fungi belonging to basidiomycota. Diversity of a mating type in lentinula edodes and mating. Diversity of a mating type in lentinula edodes has been assessed by analysis of a mating loci in 127 strains collected from east asia.
Hydrophobins play important roles in morphogenesis and pathogenesis in fungi and fruit development in mushrooms. A genome survey and senescence transcriptome analysis in lentinula edodes. Release of genome sequence data of shiitake mushroom. Pegler is a tetrapolar basidiomycete with two unlinked matingtype loci, commonly called the a and b loci.
Component name genome accessions component representation proteins. Pdf genome sequence and genetic linkage analysis of. Rapid genotyping by lowcoverage resequencing to construct. Shiitakegdb includes genome assemblies, annotation of genes, repeats, heterozygous snps, trna, mirnas, full length cdna, transcriptome data for lentinula edodes. Studies on protoplast fusion between lentinula edodes and. Genome sequence of the edible cultivated mushroom lentinula edodes.
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